II. Materials and experimental methods
1. Food waste
Reducing Sugar Production using Food Waste Food waste used in this study, Kangwon National University It was gathered that occurred in the cafeteria, household blender (SMKA (N) -4000, PN often, Republic of Korea) after crushing to 105oC Dry. The dry mix food waste After 4 mm sieve was used in the filter experiments by crushing together
Reducing Sugar Production using Food Waste Food waste used in this study, Kangwon National University It was gathered that occurred in the cafeteria, household blender (SMKA (N) -4000, PN often, Republic of Korea) after crushing to 105oC Dry. The dry mix food waste After 4 mm sieve was used in the filter experiments by crushing together
2. Food waste enzymatic hydrolysis
Reducing Sugar Production using Food Waste Korea enzymes for hydrolysis of food waste The company was purchased from Novozymes, used as a Viscozyme L. This enzyme complex as a multi-enzyme is derived from Aspergillus aculeatus Standing carbohydrates, araban, cellulose, β-glucan, hemicellulose, A hydrolase xylan degrading to 9). Optimum pH 3.3 ~ 5.5 Gravity 1.2, the maximum temperature 22 ~ 55oC, special, 100 FBG / g of activity (Table 2) 1,7). 1 FBG is the standard State (30oC, pH 5.0, reaction time 30 minutes) 1 μmol per minute This means the amount of glucose and carbohydrates produce the enzyme 7 shows), which can decompose to reduce power.Reducing Sugar Production using Food Waste
A batch reactor 200 g, dry food waste Viscozyme L 10 mm (1,200 FBG), 190 ml of buffer solution (acetic acid buffer solution) was added to the shaking incubator (VS-8480, led by the Vision Science, Republic of Korea) 150 RPM Temperature 35 ~ 55oC, the reaction mixture while mixing at 9 ~ 15 hours This range has been hydrolyzed. After the end of hydrolysis 30 ml sample of 3,000 RPM, centrifuged to obtain 5 bungan A reducing sugar was measured supernatant.Reducing Sugar Production using Food Waste
A batch reactor 200 g, dry food waste Viscozyme L 10 mm (1,200 FBG), 190 ml of buffer solution (acetic acid buffer solution) was added to the shaking incubator (VS-8480, led by the Vision Science, Republic of Korea) 150 RPM Temperature 35 ~ 55oC, the reaction mixture while mixing at 9 ~ 15 hours This range has been hydrolyzed. After the end of hydrolysis 30 ml sample of 3,000 RPM, centrifuged to obtain 5 bungan A reducing sugar was measured supernatant.Reducing Sugar Production using Food Waste
3. Analysis Method
3,5-dinitrosalicylic acid (DNS) method to reduce the 10) Sugar by side War intake, by coloring with DNS resolution and Rochelle salt A quantitative analysis of the measured light intensity. Hydrolysis end The saccharification solid solution using a glass fiber filter paper (GF-C) After removing the saccharification liquid was diluted with 3 ml of DNS reagent water A mixture of 3ML Heater (HS-R200, Humas, Republic of Korea) Rochelle salt in a 1 ml of the reaction for 5 minutes at 90oC To the bus was allowed to cool to room temperature in a room there. Analysis room Using a 575 nm wavelength UV spectrophotometer Act It was measured at a concentration of glucose in the 0.2 ~ 1.6mg / ml A calibration curve after the exchange of samples Was the amount of raw sugar.Reducing Sugar Production using Food Waste
3,5-dinitrosalicylic acid (DNS) method to reduce the 10) Sugar by side War intake, by coloring with DNS resolution and Rochelle salt A quantitative analysis of the measured light intensity. Hydrolysis end The saccharification solid solution using a glass fiber filter paper (GF-C) After removing the saccharification liquid was diluted with 3 ml of DNS reagent water A mixture of 3ML Heater (HS-R200, Humas, Republic of Korea) Rochelle salt in a 1 ml of the reaction for 5 minutes at 90oC To the bus was allowed to cool to room temperature in a room there. Analysis room Using a 575 nm wavelength UV spectrophotometer Act It was measured at a concentration of glucose in the 0.2 ~ 1.6mg / ml A calibration curve after the exchange of samples Was the amount of raw sugar.Reducing Sugar Production using Food Waste
4. Design of Experiments
The experimental design used in the experiment is estimated to be the second model Custom algorithms that are commonly used in research on The response surface methodology (response surface method) This comprehensive design (composit was applied on the design). of the Core composite design (Formula 1) are preferred features of experimental design Block Orthogonal easy to implement and chatty two car models In order to effectively evaluate the anti-sq 11) is possible. Initial experiments Temperature 35 ~ 55oC, reaction time in the range of 9 ~ 15 hours 2 stand factors (temperature, reaction time) was designed.
The experimental design used in the experiment is estimated to be the second model Custom algorithms that are commonly used in research on The response surface methodology (response surface method) This comprehensive design (composit was applied on the design). of the Core composite design (Formula 1) are preferred features of experimental design Block Orthogonal easy to implement and chatty two car models In order to effectively evaluate the anti-sq 11) is possible. Initial experiments Temperature 35 ~ 55oC, reaction time in the range of 9 ~ 15 hours 2 stand factors (temperature, reaction time) was designed.